Journal: Clinical and Translational Radiation Oncology

Article Title: Differential regulation of radioadaptation by quercetin between human normal and cancer cells

doi: 10.1016/j.ctro.2025.101099

Figure Lengend Snippet: Quercetin restores NRF2 nuclear translocation in radioadapted MCF10A cells. Representative immunofluorescence images of NRF2 (green) and DAPI (blue) and quantification of the nuclear-to-cytoplasmic NRF2 fluorescence ratio in MCF10A cells 24 h after 5 Gy irradiation with or without prior LDRT and quercetin treatment (Scale bar: 10 μm). Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were incubated with rabbit anti-human NRF2 primary antibody (1:200; Proteintech, 16396–1-AP), and detection was performed using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Translocation Assay, Immunofluorescence, Fluorescence, Irradiation

Journal: Non-coding RNA Research

Article Title: MiR-21 modulates P.g- LPS induced apoptosis and inflammatory response in HUVECs via NF-κB/iNOS/NO pathway by targeting PDCD4

doi: 10.1016/j.ncrna.2025.10.001

Figure Lengend Snippet: Dose-and time-dependent induction of inflammation and apoptosis in HUVECs following P.g- LPS exposure. (a) CCK-8 assay demonstrating decreased cell viability after 12, 24, and 32-h incubation with P.g- LPS at concentrations ranging from 0 μg/mL to 1 μg/mL. (b–c) ELISA quantification of IL-6 and TNF-α in supernatants. (d–e) Western blot analysis showing increased expression of apoptotic markers Bax and cleaved caspase-3. β-actin was used as a loading control. (f–g) Immunofluorescence showed an increase in P.g -LPS and a decrease in the expression of tight junction protein ZO-1. All data are presented as the mean ± SD. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. Abbreviations: HUVECs = Human umbilical vein endothelial cells; P.g -LPS = Porphyromonas gingivalis lipopolysaccharide; CCK-8 = Cell Counting Kit-8; IL-6 = Interleukin-6; TNF-α = Tumor necrosis factor-alpha; ZO-1 = Zonula Occludens-1; SD = Standard deviation.

Article Snippet: The coverslips with cells were incubated with goat anti-human Zonula Occludens-1 (ZO-1) primary antibody (Proteintech, 21773-1-AP, 1:200) overnight at 4 °C.

Techniques: CCK-8 Assay, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control, Immunofluorescence, Cell Counting, Standard Deviation

Journal: Translational Cancer Research

Article Title: OX40L and IL-2 combination strategy for gastric cancer immunotherapy

doi: 10.21037/tcr-2025-707

Figure Lengend Snippet: Immunohistochemical and flow cytometric analysis of gastric cancer tissue. (A) H&E histochemical staining of tumor-free area (left), tumor margin (middle), and gastric tumor site (right). (B) Representative IHC images for CD3 + , CD4 + , CD8 + , FOXP3, OX40, and OX40L in tumor-free area (left column), tumor margin (middle column), and gastric tumor site (right column) sections. (C) Immunofluorescence staining for the co-expression of CD3 + and OX40 in gastric cancer with anti-CD3 and anti-OX40 antibodies and DAPI for nuclear staining. (D) Flow cytometry analysis of OX40 expression in CD3 cells obtained from PBMC of gastric cancer patients (upper panel) and OX40 expression on CD3 on TILs in gastric cancer patients (low panel). Statistical analysis of all data were performed using one-way ANOVA. Data are shown as mean ± standard deviation. ns, nonsignificant; *, P<0.05; ***, P<0.001. ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole; H&E, hematoxylin & eosin; IHC, immunohistochemistry; OX40L, OX40 ligand; PBMC, peripheral blood mononuclear cells; TILs, tumor-infiltrating lymphocytes.

Article Snippet: Immunohistochemistry (IHC) analyses were performed using specific primary rabbit anti-human CD3 monoclonal antibody (Proteintech Group, Wuhan, China), mouse anti-human OX40 and rabbit anti-human OX40L monoclonal antibodies (CST, Danvers, MA, USA), and rabbit anti-human CD4 (Proteintech Group, Wuhan, China), mouse anti-human CD8 (Proteintech Group, Wuhan, China) antibody, rabbit anti-human FOXP3 monoclonal antibodies (CST, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Staining, Immunofluorescence, Expressing, Flow Cytometry, Standard Deviation, Immunohistochemistry

Journal: Translational Cancer Research

Article Title: Prognostic chromatin remodeling signature stratifies survival outcomes in lung adenocarcinoma patients

doi: 10.21037/tcr-2025-1699

Figure Lengend Snippet: GJB3 knockdown impairs the oncogenic behavior of LUAD cells. (A,B) Efficient knockdown of GJB3 was confirmed by Western blot and qRT-PCR in A549 and H1299 cells. (C) The proliferative capacity of cells was significantly reduced after GJB3 silencing, as measured by CCK‑8 assay. (D,E) GJB3 knockdown markedly attenuated the migration and invasion capabilities of LUAD cells, shown by representative Transwell images (stained with crystal violet) at 200× magnification (D) and their quantification (E). All data are shown as mean ± SD (n=3). ****, P<0.0001. CCK-8, Cell Counting Kit-8; LUAD, lung adenocarcinoma; OD, optical density; qRT-PCR, quantitative reverse transcription polymerase chain reaction; SD, standard deviation.

Article Snippet: The membranes were blocked with 5% skim milk and then incubated overnight at 4 °C with rabbit anti-human GJB3 primary antibody (1:1,000, Proteintech, 12880-1-AP) and mouse anti-human GAPDH primary antibody (1:5,000, Proteintech, 60004-1-Ig).

Techniques: Knockdown, Western Blot, Quantitative RT-PCR, CCK-8 Assay, Migration, Staining, Cell Counting, Reverse Transcription, Polymerase Chain Reaction, Standard Deviation